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pSG5-HA-ERR3 S57,81,219A
(Plasmid #37850)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 37850 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pSG5
  • Backbone size w/o insert (bp) 4100
  • Total vector size (bp) 5516
  • Modifications to backbone
    Modifications to Backbone: HA tag and modified multiple cloning site introduced. Original reference for pSG5-HA: PMID 10381882
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL10 Gold
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Estrogen-related receptor gamma
  • Alt name
    ERRg, ERR3
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1416
  • Mutation
    changed Serines 57, 81, and 219 to Alanine (AGC to GCC for Ser57 and Ser219; TCG to GCG for Ser81)
  • Entrez Gene
    Esrrg (a.k.a. ERR3, Errg, NR3B3, mKIAA0832)
  • Promoter SV40
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (destroyed during cloning)
  • 3′ cloning site BglII (destroyed during cloning)
  • 5′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The original, wild type pSG5-HA-ERR3 construct was generated by Dr. Michael Stallcup. Original reference: PMID 10428842
  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSG5-HA-ERR3 S57,81,219A was a gift from Rebecca Riggins (Addgene plasmid # 37850 ; http://n2t.net/addgene:37850 ; RRID:Addgene_37850)
  • For your References section:

    ERK/MAPK regulates ERRgamma expression, transcriptional activity, and receptor-mediated Tamoxifen resistance in ER+ breast cancer. Heckler MM, Thakor H, Schafer CC, Riggins RB. FEBS J. 2014 Mar 29. doi: 10.1111/febs.12797. 10.1111/febs.12797 PubMed 24684682