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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 38056 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepXCXCMV
- Total vector size (bp) 8715
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Vector typeMammalian Expression, Adenoviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nametTA
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Alt nametet transactivator
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Adenoviral plasmid for overexpression of tet transactivator (tTA) from CMV promoter
Addgene's sequencing result identified an 82bp insert at bp# 699 when compared to the full plasmid sequence provided by the depositing laboratory. This difference does not affect the function of the plasmid.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pXCX.CMV.tTA was a gift from James Uney (Addgene plasmid # 38056 ; http://n2t.net/addgene:38056 ; RRID:Addgene_38056) -
For your References section:
Increased utility in the CNS of a powerful neuron-specific tetracycline-regulatable adenoviral system developed using a post-transcriptional enhancer. Lee YB, Cosgrave AS, Glover CP, Bienemann A, Heywood D, Hobson RJ, Uney JB. J Gene Med. 2005 May;7(5):576-83. 10.1002/jgm.694 PubMed 15580589
