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pET-His6-ERK2-MEK1_R4F_coexpression
(Plasmid #39212)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 39212 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    Mek1 R4F
  • Species
    H. sapiens (human)
  • Mutation
    MEK1(R4F) = S218E, S222D and deletion of AA32-51
  • Entrez Gene
    MAP2K1 (a.k.a. CFC3, MAPKK1, MEK1, MKK1, PRKMK1)
  • Promoter T7
  • Tag / Fusion Protein
    • RBS (C terminal on backbone)

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site NA (unknown if destroyed)
  • 3′ cloning site NA (unknown if destroyed)
  • 5′ sequencing primer T7
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    Erk2
  • Species
    R. norvegicus (rat)
  • Entrez Gene
    Mapk1 (a.k.a. ERK-2, ERT1, Erk2, p42-MAPK)
  • Promoter T7 via RBS
  • Tag / Fusion Protein
    • His6 (N terminal on insert)

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site NA (unknown if destroyed)
  • 3′ cloning site NA (unknown if destroyed)
  • 5′ sequencing primer none
  • 3′ sequencing primer rErk2-R
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-His6-ERK2-MEK1_R4F_coexpression was a gift from Melanie Cobb (Addgene plasmid # 39212 ; http://n2t.net/addgene:39212 ; RRID:Addgene_39212)
  • For your References section:

    Reconstitution of mitogen-activated protein kinase phosphorylation cascades in bacteria. Efficient synthesis of active protein kinases. Khokhlatchev A, Xu S, English J, Wu P, Schaefer E, Cobb MH. J Biol Chem. 1997 Apr 25;272(17):11057-62. 10.1074/jbc.272.17.11057 PubMed 9110999