Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||39230||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeBacterial Expression
Growth in Bacteria
SpeciesR. norvegicus (rat)
Entrez GeneMapk1 (a.k.a. ERK-2, ERT1, Erk2, p42-MAPK)
- Promoter T7
/ Fusion Protein
- His (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:NpT7-5-Erk2 was a gift from Melanie Cobb (Addgene plasmid # 39230 ; http://n2t.net/addgene:39230 ; RRID:Addgene_39230)
For your References section:Regulation and properties of extracellular signal-regulated protein kinases 1 and 2 in vitro. Robbins DJ, Zhen E, Owaki H, Vanderbilt CA, Ebert D, Geppert TD, Cobb MH. J Biol Chem. 1993 Mar 5;268(7):5097-106. PubMed 8444886