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(Plasmid #39993)


Item Catalog # Description Quantity Price (USD)
Plasmid 39993 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 3000
  • Total vector size (bp) 5200
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin (50 ug/mL)
  • Growth Temperature
  • Growth Strain(s)
    JC4588 lambda+
  • Growth instructions
    Induce expression by transfer to 37˚C.
  • Copy number


  • Gene/Insert name
    EcoRI endonuclease & EcoRI methylase
  • Species
    E. coli
  • Insert Size (bp)
  • Mutation
  • GenBank ID
  • Promoter n/a
  • Tag / Fusion Protein
    • none

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HpaI (unknown if destroyed)
  • 3′ cloning site HpaI (unknown if destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer n/a
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pKC30 plasmid derivation is described in Nature (1981) 292, 128-132.

Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSCC2 was a gift from Paul Modrich (Addgene plasmid # 39993 ; ; RRID:Addgene_39993)
  • For your References section:

    Isolation of gram quantities of EcoRI restriction and modification enzymes from an overproducing strain. Cheng SC, Kim R, King K, Kim SH, Modrich P. J Biol Chem. 1984 Sep 25;259(18):11571-5. PubMed 6088551