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pUC57 APEX
(Plasmid #40306)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 40306 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pUC57
  • Backbone manufacturer
    Genscript
  • Backbone size w/o insert (bp) 2710
  • Total vector size (bp) 3457
  • Vector type
    Unspecified

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    pea APEX (Synthetic)
  • Alt name
    pea APX with K14D, W41F, E112K mutations
  • Species
    Synthetic
  • Insert Size (bp)
    747
  • Mutation
    K14D, W41F, E112K (relative to wild-type)

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer pUC57 Forward (GTAAAACGACGGCCAGTG)
  • 3′ sequencing primer pUC57 Reverse (GGAAACAGCTATGACCATG)
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

This gene encodes APEX, a monomeric and improved-activity variant of pea ascorbate peroxidase. This plasmid is not for mammalian expression and should instead be used for PCR amplification of the APEX insert. The gene is mammalian codon optimized.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUC57 APEX was a gift from Alice Ting (Addgene plasmid # 40306 ; http://n2t.net/addgene:40306 ; RRID:Addgene_40306)
  • For your References section:

    Engineered ascorbate peroxidase as a genetically encoded reporter for electron microscopy. Martell JD, Deerinck TJ, Sancak Y, Poulos TL, Mootha VK, Sosinsky GE, Ellisman MH, Ting AY. Nat Biotechnol. 2012 Oct 21. doi: 10.1038/nbt.2375. 10.1038/nbt.2375 PubMed 23086203