Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Addgene is open and shipping orders.

  • Pending order? We will email to confirm that your organization can accept shipments.
  • Conducting coronavirus or COVID-19 research? Email us at [email protected] with your order or deposit number so we can prioritize it.

Learn more about our current shipping status and COVID-19 resources.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pDEST47-Arl13b12
(Plasmid #40872)

Loading...

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 40872 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA-DEST47
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 7780
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Arl13b
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1410
  • Mutation
    no stop codon
  • Entrez Gene
    Arl13b (a.k.a. A530097K21Rik, A930014M17Rik, Arl2l1, C530009C10Rik, hnn)
  • Promoter CMV
  • Tag / Fusion Protein
    • GFP (C terminal on backbone)

Cloning Information

Resource Information

Depositor Comments

In Larkins et al (2011), this is plasmid "Arl13b GFP tagged" as referenced in Figure 2. Full-length Arl13b without a stop codon (pENTR-Arl13b2) was Gateway cloned into pcDNA-DEST47.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDEST47-Arl13b12 was a gift from Tamara Caspary (Addgene plasmid # 40872 ; http://n2t.net/addgene:40872 ; RRID:Addgene_40872)
  • For your References section:

    Arl13b regulates ciliogenesis and the dynamic localization of Shh signaling proteins. Larkins CE, Aviles GD, East MP, Kahn RA, Caspary T. Mol Biol Cell. 2011 Dec;22(23):4694-703. Epub 2011 Oct 5. 10.1091/mbc.E10-12-0994 PubMed 21976698