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pLEX_307
(Plasmid #41392)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 41392 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pLKO
  • Backbone size (bp) 10065
  • Vector type
    Mammalian Expression, Lentiviral ; Gateway Destination vector
  • Promoter EF1a
  • Selectable markers
    Puromycin
  • Tag / Fusion Protein
    • V5 (C terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin
  • Growth Temperature
    30°C
  • Growth Strain(s)
    ccdB Survival
  • Growth instructions
    ccdB resistant bacteria. 30C for liquid culture. 37C for plates.
  • Copy number
    High Copy

Cloning Information

Resource Information

Depositor Comments

Plasmid for constitutive lentiviral expression.

There is a C-terminal V5 tag followed by a stop codon directly after the Gateway sequence.

Alternate plasmid names:
pLX307,
SV40-puro; EF1a-gateway-V5 tag

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLEX_307 was a gift from David Root (Addgene plasmid # 41392 ; http://n2t.net/addgene:41392 ; RRID:Addgene_41392)