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pcDNA3 βarr1 S412A Flag
(Plasmid #42195)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 42195 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5446
  • Total vector size (bp) 6700
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    β-arrestin 1 S412A
  • Alt name
    β-arrestin 1
  • Alt name
    Beta arrestin 1
  • Alt name
    arrb1
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    1300
  • Mutation
    S412A
  • GenBank ID
    NM_012910.2 NP_037042.1
  • Entrez Gene
    Arrb1 (a.k.a. BARRES)
  • Promoter CMV
  • Tag / Fusion Protein
    • Flag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Flag epitope-tagged truncation mutants of β-arrestin 1 were prepared by the polymerase chain reaction (PCR), incorporating an Eco RI site, minimal Kozak sequence (ACC), and initiator methionine codon into the 5' primer, and the Flag epitope sequence, stop codon, and an Xho I site into the 3' primer. PCR products were subcloned into pcDNA3. DNA sequences were confirmed by dideoxynucleotide sequencing.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3 βarr1 S412A Flag was a gift from Robert Lefkowitz (Addgene plasmid # 42195 ; http://n2t.net/addgene:42195 ; RRID:Addgene_42195)
  • For your References section:

    Beta-arrestin-dependent formation of beta2 adrenergic receptor-Src protein kinase complexes. Luttrell LM, Ferguson SS, Daaka Y, Miller WE, Maudsley S, Della Rocca GJ, Lin F, Kawakatsu H, Owada K, Luttrell DK, Caron MG, Lefkowitz RJ. Science. 1999 Jan 29. 283(5402):655-61. 10.1126/science.283.5402.655 PubMed 9924018