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pcDNA3-GI-ZFP2
(Plasmid #42215)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 42215 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5334
  • Total vector size (bp) 9276
  • Modifications to backbone
    ZFP2 was inserted into the GI-ZFP1 plasmid using the NotI and XbaI sites to create the GI-FLAG-ZFP2 gene. The FLAG tag was then inserted using the NotI site.

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GIGANTEA-FLAG-ZFP2
  • Alt name
    GI-FLAG-ZFP2
  • Alt name
    GI-ZFP2
  • Alt name
    GI
  • Species
    A. thaliana (mustard weed), Synthetic
  • Insert Size (bp)
    3942
  • Promoter CMV
  • Tag / Fusion Protein
    • FLAG (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer ATTGACGCAAATGGGCGGTAGGCGTGT
  • 3′ sequencing primer GCC TGC TAT TGT CTT CCC AAT
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-GI-ZFP2 was a gift from Charles Gersbach (Addgene plasmid # 42215)
  • For your References section:

    Light-inducible spatiotemporal control of gene activation by customizable zinc finger transcription factors. Polstein LR, Gersbach CA. J Am Chem Soc. 2012 Oct 10;134(40):16480-3. doi: 10.1021/ja3065667. Epub 2012 Sep 27. 10.1021/ja3065667 PubMed 22963237