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MLM3636
(Plasmid #43860)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 43860 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    Human-gRNA-ExpressionVector
  • Backbone manufacturer
    Joung lab MLM3636
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    None
  • Promoter U6

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsmBI (not destroyed)
  • 3′ cloning site BsmBI (not destroyed)
  • 5′ sequencing primer OS280 (5’-CAGGGTTATTGTCTCATGAGCGG-3’)
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Human cell gRNA Expression Vector for use with JDS246 (Addgene plasmid# 43861--mammalian codon-optimized streptococcus pyogenes Cas9). See the supplemental document "Joung Lab gRNA Cloning Protocol" for more details.

For more information on Joung Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/jounglab/

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MLM3636 was a gift from Keith Joung (Addgene plasmid # 43860)