Purposeguide RNA (gRNA) expression vector used to create a gRNA to a specific sequence, uses U6 promoter
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||43860||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerJoung lab MLM3636
Vector typeMammalian Expression, CRISPR
Growth in Bacteria
Growth Strain(s)XL1 Blue
- Promoter U6
- Cloning method Restriction Enzyme
- 5′ cloning site BsmBI (not destroyed)
- 3′ cloning site BsmBI (not destroyed)
- 5′ sequencing primer OS280 (5’-CAGGGTTATTGTCTCATGAGCGG-3’) (Common Sequencing Primers)
Human cell gRNA Expression Vector for use with JDS246 (Addgene plasmid# 43861--mammalian codon-optimized streptococcus pyogenes Cas9). See the supplemental document "Joung Lab gRNA Cloning Protocol" for more details.
For more information on Joung Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/jounglab/
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:MLM3636 was a gift from Keith Joung (Addgene plasmid # 43860 ; http://n2t.net/addgene:43860 ; RRID:Addgene_43860)