pDEST-IRES-puro-polyA
(Plasmid #43967)

• Depositing Lab: Peter Hohenstein
• Publication: Dolt et al PLoS One. 2013 Apr 29;8(4):e62054. doi: 10.1371/journal.pone.0062054. Print 2013.

Backbone

• Vector backbone: PL451
• Backbone size w/o insert (bp): 7423
• Total vector size (bp): 8746
• Vector type: Gateway DEST vector
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Kanamycin, 25 & 50 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): ccdB Survival
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: puroR
• Alt name: puromycin -R
• Alt name: PAC
• Insert Size (bp): 600

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: n/a
• 3′ sequencing primer: n/a

Resource Information

• Supplemental Documents:
  • full sequence in Geneious format
  • full sequence in genbank format

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Note that this plasmid also confers Amp resistance.

There are some discrepancies between Addgene's quality control sequence and the full assembled sequence from the depositor. These discrepancies should not affect plasmid function.