|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||45434||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeSynthetic Biology
Growth in Bacteria
Growth Strain(s)DH1 with AcrAB knocked out
Copy numberLow Copy
Gene/Insert nameAcrB/AcrD/AcrFa, ABO_0964
Insert Size (bp)5927
- Promoter LacUV5
/ Fusion Protein
- His (C terminal on insert)
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer CAA AAG ATC TTT TAA GAA GGA GAT ATA CAT ATG TAT ATA CTG AAC CTG ATT CA
- 3′ sequencing primer CAG TGG TGA TGG TGA TGA TGG CGG CTG GCC ACA TGG GTG GGC (Common Sequencing Primers)
Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBbA5k-EPL95 was a gift from Aindrila Mukhopadhyay (Addgene plasmid # 45434 ; http://n2t.net/addgene:45434 ; RRID:Addgene_45434)
For your References section:Engineering microbial biofuel tolerance and export using efflux pumps. Dunlop MJ, Dossani ZY, Szmidt HL, Chu HC, Lee TS, Keasling JD, Hadi MZ, Mukhopadhyay A. Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21. 10.1038/msb.2011.21 PubMed 21556065