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pKH3-TRIM28
(Plasmid #45569)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 45569 Standard format: Plasmid sent in bacteria as agar stab 1 $75 *

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Backbone

  • Vector backbone
    pKH3
  • Backbone manufacturer
    Addgene plasmid Plasmid 12555
  • Backbone size w/o insert (bp) 4800
  • Total vector size (bp) 7300
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TRIM28
  • Alt name
    tripartite motif containing 28
  • Alt name
    KAP1
  • Alt name
    TIF1β
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2500
  • GenBank ID
    NM_005762.2 NP_005753.1
  • Entrez Gene
    TRIM28 (a.k.a. KAP1, PPP1R157, RNF96, TF1B, TIF1B)
  • Promoter CMV
  • Tag / Fusion Protein
    • 3xHA (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (destroyed during cloning)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer LNCX; CMV-F
  • 3′ sequencing primer SV40-pA-R
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Full-length human TRIM28 was purchased from Origene and subcloned into vector pKH3 to generate pKH3-Trim28.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKH3-TRIM28 was a gift from Fanxiu Zhu (Addgene plasmid # 45569 ; http://n2t.net/addgene:45569 ; RRID:Addgene_45569)
  • For your References section:

    Tripartite motif-containing protein 28 is a small ubiquitin-related modifier E3 ligase and negative regulator of IFN regulatory factor 7. Liang Q, Deng H, Li X, Wu X, Tang Q, Chang TH, Peng H, Rauscher FJ 3rd, Ozato K, Zhu F. J Immunol. 2011 Nov 1;187(9):4754-63. doi: 10.4049/jimmunol.1101704. Epub 2011 Sep 21. 10.4049/jimmunol.1101704 PubMed 21940674