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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 45597 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA DEST40
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 7143
- Total vector size (bp) 6841
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namec-Myc
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Alt nameMyc2
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Alt namemyelocytomatosis oncogene
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SpeciesM. musculus (mouse)
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Insert Size (bp)1400
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Entrez GeneMyc (a.k.a. Myc2, Niard, Nird, bHLHe39)
- Promoter CMV
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Tags
/ Fusion Proteins
- V5 (C terminal on backbone)
- 6xHis (C terminal on backbone)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer CMV-F; T7
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byMurine c-myc cDNA amplified from plasmid CMV-Myc from Stephen R. Hann (Vanderbuilt University) (PMID: 7958908) (Murine myc cDNA originally obtained from Michael Cole, Princeton, NJ)
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Alternate plasmid names: pD40-His-c-Myc; pcDNA-DEST40 Myc wt
This His6-tagged c-Myc plasmid was created by PCR amplification of the coding sequence for wild-type murine c-Myc2 from the CMVMyc plasmid (Hann et al., Genes Dev. 1994, PMID: 7958908) with a CACC 5′ extension added to the 5′ primer for cloning into the TOPO entry clone from Invitrogen (Carlsbad, CA). This sequence was then moved into the pDEST-40 mammalian expression vector using Gateway technology (Invitrogen), creating pD40-His-c-Myc.
For plasmid usage information, see the associated article (Yeh et al. Nat Cell Biol. 2004) as well as Arnold et al. EMBO 2009 (PMID: 19131971).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pD40-His/V5-c-Myc was a gift from Rosalie Sears (Addgene plasmid # 45597 ; http://n2t.net/addgene:45597 ; RRID:Addgene_45597) -
For your References section:
A signalling pathway controlling c-Myc degradation that impacts oncogenic transformation of human cells. Yeh E, Cunningham M, Arnold H, Chasse D, Monteith T, Ivaldi G, Hahn WC, Stukenberg PT, Shenolikar S, Uchida T, Counter CM, Nevins JR, Means AR, Sears R. Nat Cell Biol. 2004 Apr;6(4):308-18. Epub 2004 Mar 14. 10.1038/ncb1110 PubMed 15048125