pCM130
(Plasmid #45828)

• Purpose: (Empty Backbone)
• Depositing Lab: Mary Lidstrom
• Publication: Marx et al Microbiology. 2001 Aug;147(Pt 8):2065-75.

Backbone

• Vector backbone: pCM62
• Backbone manufacturer: Lidstrom Lab (Addgene plasmid 45826)
• Backbone size (bp): 7965
• Modifications to backbone: created by inserting a 1.0 kb fragment containing xylE (from pCM75) into pCM62 (Addgene plasmid 45826)cut with AflIII and HindIII, followed by the insertion of a 0.5 kb XbaI-XmnI trrnB fragment from pMTL20T1T2 into the blunted EcoRI site.
• Vector type: low-background broad-host-range promoter-probe vector using xylE as a reporter gene
• Promoter: none

Growth in Bacteria

• Bacterial Resistance(s): Tetracycline, 10 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: M13-F20

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

XylE replaces the lac promoter in pCM62 (Addgene plasmid 45826) and is used as a reporter to test promoter activity. A transcriptional terminator was added to reduce background activity.