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pCM130
(Plasmid #45828)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 45828 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCM62
  • Backbone manufacturer
    Lidstrom Lab (Addgene plasmid 45826)
  • Backbone size (bp) 7965
  • Modifications to backbone
    created by inserting a 1.0 kb fragment containing xylE (from pCM75) into pCM62 (Addgene plasmid 45826)cut with AflIII and HindIII, followed by the insertion of a 0.5 kb XbaI-XmnI trrnB fragment from pMTL20T1T2 into the blunted EcoRI site.
  • Vector type
    low-background broad-host-range promoter-probe vector using xylE as a reporter gene
  • Promoter none

Growth in Bacteria

  • Bacterial Resistance(s)
    Tetracycline
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Cloning Information

Resource Information

  • Terms and Licenses

Depositor Comments

XylE replaces the lac promoter in pCM62 (Addgene plasmid 45826) and is used as a reporter to test promoter activity. A transcriptional terminator was added to reduce background activity.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCM130 was a gift from Mary Lidstrom (Addgene plasmid # 45828 ; http://n2t.net/addgene:45828 ; RRID:Addgene_45828)
  • For your References section:

    Development of improved versatile broad-host-range vectors for use in methylotrophs and other Gram-negative bacteria. Marx CJ, Lidstrom ME. Microbiology. 2001 Aug;147(Pt 8):2065-75. 10.1099/00221287-147-8-2065 PubMed 11495985