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pU6-BbsI-chiRNA
(Plasmid #45946)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 45946 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBS-SK(+)
  • Backbone manufacturer
    Agilent
  • Backbone size w/o insert (bp) 2958
  • Total vector size (bp) 3458
  • Vector type
    Insect Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    U6-BbsI-chiRNA
  • Alt name
    U6-SpchiRNA
  • Alt name
    CRISPR chiRNA
  • Insert Size (bp)
    500
  • Promoter Dm-snRNA:U6:96Ab

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRV (destroyed during cloning)
  • 3′ cloning site EvoRV (destroyed during cloning)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T3
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Feng Zhang, Broad Institute, MIT
  • Terms and Licenses
  • Articles Citing this Plasmid

Depositor Comments

For more information on FlyCRISPR Plasmids please refer to: http://www.addgene.org/crispr/OConnor-Giles/

Plasmid 51019: pDsRed-attP (www.addgene.org/51019) can be for generating dsDNA donors for homology-directed repair to replace genes or other genomic sequence with an attP docking site.

Please note the F1 ori is in the (+) orientation, rather than the (-) orientation shown in the assembled full sequence.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pU6-BbsI-chiRNA was a gift from Melissa Harrison & Kate O'Connor-Giles & Jill Wildonger (Addgene plasmid # 45946)
  • For your References section:

    Genome engineering of Drosophila with the CRISPR RNA-guided Cas9 nuclease. Gratz SJ, Cummings AM, Nguyen JN, Hamm DC, Donohue LK, Harrison MM, Wildonger J, O'Connor-Giles KM. Genetics. 2013 May 24. 10.1534/genetics.113.152710 PubMed 23709638