PurposePlasmid for expression of chiRNA under the control of the Drosophila snRNA:U6:96Ab promoter.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||45946||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 2958
- Total vector size (bp) 3458
Vector typeInsect Expression, CRISPR
Growth in Bacteria
Copy numberHigh Copy
Alt nameCRISPR chiRNA
Insert Size (bp)500
- Promoter Dm-snRNA:U6:96Ab
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRV (destroyed during cloning)
- 3′ cloning site EvoRV (destroyed during cloning)
- 5′ sequencing primer T7
- 3′ sequencing primer T3 (Common Sequencing Primers)
For more information on FlyCRISPR Plasmids please refer to: http://www.addgene.org/crispr/OConnor-Giles/
Plasmid 51019: pDsRed-attP (www.addgene.org/51019) can be for generating dsDNA donors for homology-directed repair to replace genes or other genomic sequence with an attP docking site.
Please note the F1 ori is in the (+) orientation, rather than the (-) orientation shown in the assembled full sequence.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pU6-BbsI-chiRNA was a gift from Melissa Harrison & Kate O'Connor-Giles & Jill Wildonger (Addgene plasmid # 45946)
For your References section:Genome engineering of Drosophila with the CRISPR RNA-guided Cas9 nuclease. Gratz SJ, Cummings AM, Nguyen JN, Hamm DC, Donohue LK, Harrison MM, Wildonger J, O'Connor-Giles KM. Genetics. 2013 May 24. 10.1534/genetics.113.152710 PubMed 23709638