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pBS-Hsp70-Cas9
(Plasmid #46294)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 46294 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBluescript-KS(+)
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 2958
  • Vector type
    Insect Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    codon optimized Cas9
  • Alt name
    SpCas9
  • Mutation
    Silent change from C to A at bp 834 of insert
  • Promoter Hsp70
  • Tag / Fusion Protein
    • 3x Flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (unknown if destroyed)
  • 3′ cloning site NotI (unknown if destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T3
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Cas9 insert from Feng Zhang, Broad Institute, MIT
  • Terms and Licenses
  • Articles Citing this Plasmid

Depositor Comments

This plasmid was created by subcloning the Hsp70-Cas9 containing NotI fragment from Addgene plasmid 45945 in NotI digested pBluescript-KS(+).

For more information on FlyCRISPR Plasmids please refer to: http://www.addgene.org/crispr/OConnor-Giles/

Plasmid 51019: pDsRed-attP (www.addgene.org/51019) can be for generating dsDNA donors for homology-directed repair to replace genes or other genomic sequence with an attP docking site.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBS-Hsp70-Cas9 was a gift from Melissa Harrison & Kate O'Connor-Giles & Jill Wildonger (Addgene plasmid # 46294 ; http://n2t.net/addgene:46294 ; RRID:Addgene_46294)