Purpose(Empty Backbone) Bacterial targeting
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||46262||Standard format: Plasmid sent in bacteria as agar stab||1||$75 *|
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Vector typeBacterial targeting
Growth in Bacteria
Growth instructionslow copy number in pir+ E. coli strains high copy number in pir116 E. coli strains
Copy numberLow Copy
Terms and Licenses
- Zeocin® is an InvivoGen trademark.
Addgene's quality control sequence shows 1bp deletion compared to depositor's reference sequence. The mismatch is in backbone region and does not affect any of the features.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pKNOCK-Km was a gift from Mikhail Alexeyev (Addgene plasmid # 46262 ; http://n2t.net/addgene:46262 ; RRID:Addgene_46262)
For your References section:The pKNOCK series of broad-host-range mobilizable suicide vectors for gene knockout and targeted DNA insertion into the chromosome of gram-negative bacteria. Alexeyev MF. Biotechniques. 1999 May;26(5):824-6, 828. 10.2144/99265bm05 PubMed 10337469