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pEGFPC1/GgVcl 1-258 A50I mutation
(Plasmid #46271)


Item Catalog # Description Quantity Price (USD)
Plasmid 46271 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 4700
  • Total vector size (bp) 7316
  • Modifications to backbone
    vector modified to contain unique Nde, Xho sites.
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
    Vcl 1-258 (aka VD1) A50I mutation
  • Alt name
  • Alt name
  • Species
    G. gallus (chicken)
  • Mutation
    1-258 (VD1 domain) A50I mutation
  • GenBank ID
  • Entrez Gene
  • Promoter CMV
  • Tag / Fusion Protein
    • EGFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer EGFP-C
  • 3′ sequencing primer SV40 pA R
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

A50I mutation introduced into EGFP/V1-258 (Addgene plasmid 46270) by QCPCR.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFPC1/GgVcl 1-258 A50I mutation was a gift from Susan Craig (Addgene plasmid # 46271 ; ; RRID:Addgene_46271)
  • For your References section:

    A conformational switch in vinculin drives formation and dynamics of a talin-vinculin complex at focal adhesions. Cohen DM, Kutscher B, Chen H, Murphy DB, Craig SW. J Biol Chem. 2006 Jun 9;281(23):16006-15. Epub 2006 Apr 10. 10.1074/jbc.M600738200 PubMed 16608855