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pCW57.1-MAT2A
(Plasmid #100521)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 100521 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCW57.1
  • Backbone manufacturer
    Carson Thoreen at Yale University
  • Modifications to backbone
    The original pCW57.1 vector was modified: rtTA (reverse tetracycline-controlled transactivator) was replaced with tTA (tetracycline-controlled transactivator (tTA), making this vector Tet-Off. Puromycin was replaced with Blasticidin selection
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    MAT2A
  • Alt name
    AMS2
  • Alt name
    MATA2
  • Species
    H. sapiens (human)
  • Entrez Gene
    MAT2A (a.k.a. MATA2, MATII, SAMS2)
  • Promoter pTight TRE

Cloning Information

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCW57.1-MAT2A was a gift from David Sabatini (Addgene plasmid # 100521 ; http://n2t.net/addgene:100521 ; RRID:Addgene_100521)
  • For your References section:

    SAMTOR is an S-adenosylmethionine sensor for the mTORC1 pathway. Gu X, Orozco JM, Saxton RA, Condon KJ, Liu GY, Krawczyk PA, Scaria SM, Harper JW, Gygi SP, Sabatini DM. Science Vol. 358, Issue 6364, pp. 813-818, 10 Nov 2017 10.1126/science.aao3265