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pmCIT-CFPGgVcl 1-419 control 2 FRET
(Plasmid #46283)


Item Catalog # Description Quantity Price (USD)
Plasmid 46283 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    Craig lab
  • Total vector size (bp) 4700
  • Modifications to backbone
    likely modified from pEYFP-C1
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
    ECFP + Vcl 1-419
  • Alt name
  • Alt name
  • Species
    G. gallus (chicken)
  • Mutation
    aa 1-419
  • Entrez Gene
  • Promoter CMV
  • Tag / Fusion Protein
    • mCitrine (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer SV40pA-R
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Citrine proved to be a more stable FP in vivo than YFP; CIT was used to make a second generation vinculin FRET probe (first generation control is Addgene plasmid 46277).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pmCIT-CFPGgVcl 1-419 control 2 FRET was a gift from Susan Craig (Addgene plasmid # 46283 ; ; RRID:Addgene_46283)
  • For your References section:

    Coincidence of actin filaments and talin is required to activate vinculin. Chen H, Choudhury DM, Craig SW. J Biol Chem. 2006 Dec 29;281(52):40389-98. Epub 2006 Oct 29. 10.1074/jbc.M607324200 PubMed 17074767