pmCIT-CFPGgVcl 1-419 control 2 FRET
(Plasmid #46283)

• Depositing Lab: Susan Craig
• Publication: Chen et al J Biol Chem. 2006 Dec 29;281(52):40389-98. Epub 2006 Oct 29.

Backbone

• Vector backbone: pmCitrine-C1
• Backbone manufacturer: Craig lab
• Total vector size (bp): 4700
• Modifications to backbone: likely modified from pEYFP-C1
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: ECFP + Vcl 1-419
• Alt name: Vinculin
• Alt name: Vcl
• Species: G. gallus (chicken)
• Mutation: aa 1-419
• Entrez Gene: VCL (a.k.a. VINC1)
• Promoter: CMV
• Tag / Fusion Protein:
  • mCitrine (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: KpnI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: SV40pA-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Citrine proved to be a more stable FP in vivo than YFP; CIT was used to make a second generation vinculin FRET probe (first generation control is Addgene plasmid 46277).