Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||46324||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Backbone manufacturerBrown lab
- Total vector size (bp) 5243
Modifications to backboneE boxes from Dpb inserted upstream of Luc reporter in pGL3.
Vector typeMammalian Expression, Luciferase
Growth in Bacteria
Copy numberHigh Copy
SpeciesS. cerevisiae (budding yeast)
Insert Size (bp)122
Mutationfive copies of a multimerized GAL4 (5×GAL4) site (cut from pFR-luc; Invitrogen)
/ Fusion Proteins
- E boxes (C terminal on backbone)
- luciferase (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (destroyed during cloning)
- 3′ cloning site XbaI (destroyed during cloning)
- 5′ sequencing primer LucNRev (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pG4-E6s-luc was a gift from Steven Brown (Addgene plasmid # 46324 ; http://n2t.net/addgene:46324 ; RRID:Addgene_46324)
For your References section:Distinct roles of DBHS family members in the circadian transcriptional feedback loop. Kowalska E, Ripperger JA, Muheim C, Maier B, Kurihara Y, Fox AH, Kramer A, Brown SA. Mol Cell Biol. 2012 Nov;32(22):4585-94. doi: 10.1128/MCB.00334-12. Epub 2012 Sep 10. 10.1128/MCB.00334-12 PubMed 22966205
Map uploaded by the depositor.