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pDD162 (Peft-3::Cas9 + Empty sgRNA) Citations (65)

Plasmid Article: Engineering the Caenorhabditis elegans genome using Cas9-triggered homologous recombination.
Dickinson DJ, Ward JD, Reiner DJ, Goldstein B Nat Methods. 2013 Sep 1. doi: 10.1038/nmeth.2641.
PubMed Journal

Articles Citing pDD162 (Peft-3::Cas9 + Empty sgRNA)

Articles
Conditional knockouts generated by engineered CRISPR-Cas9 endonuclease reveal the roles of coronin in C. elegans neural development. Shen Z, Zhang X, Chai Y, Zhu Z, Yi P, Feng G, Li W, Ou G. Dev Cell. 2014 Sep 8;30(5):625-36. doi: 10.1016/j.devcel.2014.07.017. Epub 2014 Aug 21. PubMed
CRISPR/Cas9 Genome Editing in Caenorhabditis elegans: Evaluation of Templates for Homology-Mediated Repair and Knock-Ins by Homology-Independent DNA Repair. Katic I, Xu L, Ciosk R. G3 (Bethesda). 2015 Jun 3. pii: g3.115.019273. doi: 10.1534/g3.115.019273. PubMed

Associated Plasmids

Polymerase Theta is a key driver of genome evolution and of CRISPR/Cas9-mediated mutagenesis. van Schendel R, Roerink SF, Portegijs V, van den Heuvel S, Tijsterman M. Nat Commun. 2015 Jun 16;6:7394. doi: 10.1038/ncomms8394. PubMed
Context-dependent modulation of Pol II CTD phosphatase SSUP-72 regulates alternative polyadenylation in neuronal development. Chen F, Zhou Y, Qi YB, Khivansara V, Li H, Chun SY, Kim JK, Fu XD, Jin Y. Genes Dev. 2015 Nov 15;29(22):2377-90. doi: 10.1101/gad.266650.115. PubMed
The Stress Granule RNA-Binding Protein TIAR-1 Protects Female Germ Cells from Heat Shock in Caenorhabditis elegans. Huelgas-Morales G, Silva-Garcia CG, Salinas LS, Greenstein D, Navarro RE. G3 (Bethesda). 2016 Apr 7;6(4):1031-47. doi: 10.1534/g3.115.026815. PubMed
Neuroendocrine Coordination of Mitochondrial Stress Signaling and Proteostasis. Berendzen KM, Durieux J, Shao LW, Tian Y, Kim HE, Wolff S, Liu Y, Dillin A. Cell. 2016 Sep 8;166(6):1553-1563.e10. doi: 10.1016/j.cell.2016.08.042. PubMed
Engineering new balancer chromosomes in C. elegans via CRISPR/Cas9. Iwata S, Yoshina S, Suehiro Y, Hori S, Mitani S. Sci Rep. 2016 Sep 21;6:33840. doi: 10.1038/srep33840. PubMed
The tubulin repertoire of C. elegans sensory neurons and its context-dependent role in process outgrowth. Lockhead D, Schwarz EM, O'Hagan R, Bellotti S, Krieg M, Barr MM, Dunn AR, Sternberg PW, Goodman MB. Mol Biol Cell. 2016 Sep 21. pii: mbc.E16-06-0473. PubMed
Nuclei migrate through constricted spaces using microtubule motors and actin networks in C. elegans hypodermal cells. Bone CR, Chang YT, Cain NE, Murphy SP, Starr DA. Development. 2016 Nov 15;143(22):4193-4202. Epub 2016 Oct 3. PubMed
Neuropeptide signals cell non-autonomous mitochondrial unfolded protein response. Shao LW, Niu R, Liu Y. Cell Res. 2016 Nov;26(11):1182-1196. doi: 10.1038/cr.2016.118. Epub 2016 Oct 21. PubMed
FOS-1 functions as a transcriptional activator downstream of the C. elegans JNK homolog KGB-1. Zhang Z, Liu L, Twumasi-Boateng K, Block DH, Shapira M. Cell Signal. 2017 Jan;30:1-8. doi: 10.1016/j.cellsig.2016.11.010. Epub 2016 Nov 15. PubMed
PIGN prevents protein aggregation in the endoplasmic reticulum independently of its function in the GPI synthesis. Ihara S, Nakayama S, Murakami Y, Suzuki E, Asakawa M, Kinoshita T, Sawa H. J Cell Sci. 2016 Dec 15. pii: jcs.196717. PubMed
Analysis of postcytokinetic roles of cytokinetic components in Caenorhabditis elegans. Chai Y, Tian D, Li W, Ou G. Methods Cell Biol. 2017;137:253-266. doi: 10.1016/bs.mcb.2016.03.040. Epub 2016 Jun 1. PubMed
Tissue-specific regulation of alternative polyadenylation represses expression of a neuronal ankyrin isoform in C. elegans epidermal development. Chen F, Chisholm AD, Jin Y. Development. 2017 Feb 15;144(4):698-707. doi: 10.1242/dev.146001. Epub 2017 Jan 13. PubMed
Antidromic-rectifying gap junctions amplify chemical transmission at functionally mixed electrical-chemical synapses. Liu P, Chen B, Mailler R, Wang ZW. Nat Commun. 2017 Mar 20;8:14818. doi: 10.1038/ncomms14818. PubMed
Plastin increases cortical connectivity to facilitate robust polarization and timely cytokinesis. Ding WY, Ong HT, Hara Y, Wongsantichon J, Toyama Y, Robinson RC, Nedelec F, Zaidel-Bar R. J Cell Biol. 2017 May 1;216(5):1371-1386. doi: 10.1083/jcb.201603070. Epub 2017 Apr 11. PubMed
Twitchin kinase inhibits muscle activity. Matsunaga Y, Hwang H, Franke B, Williams R, Penley M, Qadota H, Yi H, Morran LT, Lu H, Mayans O, Benian GM. Mol Biol Cell. 2017 Jun 15;28(12):1591-1600. doi: 10.1091/mbc.E16-10-0707. Epub 2017 Apr 20. PubMed
RNA Binding Protein Vigilin Collaborates with miRNAs To Regulate Gene Expression for Caenorhabditis elegans Larval Development. Zabinsky RA, Weum B, Cui M, Han M. G3 (Bethesda). 2017 Jun 2. pii: g3.117.043414. doi: 10.1534/g3.117.043414. PubMed
Genome Editing of C. elegans. Sugi T. Methods Mol Biol. 2017;1630:247-254. doi: 10.1007/978-1-4939-7128-2_20. PubMed
Mechanism of biofilm-mediated stress resistance and lifespan extension in C. elegans. Smolentseva O, Gusarov I, Gautier L, Shamovsky I, DeFrancesco AS, Losick R, Nudler E. Sci Rep. 2017 Aug 2;7(1):7137. doi: 10.1038/s41598-017-07222-8. PubMed
Targeted genome editing in Caenorhabditis elegans using CRISPR/Cas9. Farboud B. Wiley Interdiscip Rev Dev Biol. 2017 Nov;6(6). doi: 10.1002/wdev.287. Epub 2017 Aug 15. PubMed
BORC Regulates the Axonal Transport of Synaptic Vesicle Precursors by Activating ARL-8. Niwa S, Tao L, Lu SY, Liew GM, Feng W, Nachury MV, Shen K. Curr Biol. 2017 Sep 11;27(17):2569-2578.e4. doi: 10.1016/j.cub.2017.07.013. Epub 2017 Aug 17. PubMed
LET-413/Erbin acts as a RAB-5 effector to promote RAB-10 activation during endocytic recycling. Liu H, Wang S, Hang W, Gao J, Zhang W, Cheng Z, Yang C, He J, Zhou J, Chen J, Shi A. J Cell Biol. 2018 Jan 2;217(1):299-314. doi: 10.1083/jcb.201705136. Epub 2017 Oct 27. PubMed
An Intracellular Pathogen Response Pathway Promotes Proteostasis in C. elegans. Reddy KC, Dror T, Sowa JN, Panek J, Chen K, Lim ES, Wang D, Troemel ER. Curr Biol. 2017 Nov 20;27(22):3544-3553.e5. doi: 10.1016/j.cub.2017.10.009. Epub 2017 Nov 2. PubMed
Polo-like Kinase Couples Cytoplasmic Protein Gradients in the C. elegans Zygote. Han B, Antkowiak KR, Fan X, Rutigliano M, Ryder SP, Griffin EE. Curr Biol. 2018 Jan 8;28(1):60-69.e8. doi: 10.1016/j.cub.2017.11.048. Epub 2017 Dec 21. PubMed
Tumor suppressor APC is an attenuator of spindle-pulling forces during C. elegans asymmetric cell division. Sugioka K, Fielmich LE, Mizumoto K, Bowerman B, van den Heuvel S, Kimura A, Sawa H. Proc Natl Acad Sci U S A. 2018 Jan 30;115(5):E954-E963. doi: 10.1073/pnas.1712052115. Epub 2018 Jan 18. PubMed
A Neuronal piRNA Pathway Inhibits Axon Regeneration in C. elegans. Kim KW, Tang NH, Andrusiak MG, Wu Z, Chisholm AD, Jin Y. Neuron. 2018 Feb 7;97(3):511-519.e6. doi: 10.1016/j.neuron.2018.01.014. Epub 2018 Jan 27. PubMed
TMC Proteins Modulate Egg Laying and Membrane Excitability through a Background Leak Conductance in C. elegans. Yue X, Zhao J, Li X, Fan Y, Duan D, Zhang X, Zou W, Sheng Y, Zhang T, Yang Q, Luo J, Duan S, Xiao R, Kang L. Neuron. 2018 Feb 7;97(3):571-585.e5. doi: 10.1016/j.neuron.2017.12.041. Epub 2018 Jan 27. PubMed
Genetic Suppression of Basement Membrane Defects in Caenorhabditis elegans by Gain of Function in Extracellular Matrix and Cell-Matrix Attachment Genes. Gotenstein JR, Koo CC, Ho TW, Chisholm AD. Genetics. 2018 Apr;208(4):1499-1512. doi: 10.1534/genetics.118.300731. Epub 2018 Feb 12. PubMed
MIB-1 Is Required for Spermatogenesis and Facilitates LIN-12 and GLP-1 Activity in Caenorhabditis elegans. Ratliff M, Hill-Harfe KL, Gleason EJ, Ling H, Kroft TL, L'Hernault SW. Genetics. 2018 May;209(1):173-193. doi: 10.1534/genetics.118.300807. Epub 2018 Mar 12. PubMed
SAC-1 ensures epithelial endocytic recycling by restricting ARF-6 activity. Chen D, Yang C, Liu S, Hang W, Wang X, Chen J, Shi A. J Cell Biol. 2018 Jun 4;217(6):2121-2139. doi: 10.1083/jcb.201711065. Epub 2018 Mar 21. PubMed
The kinases male germ cell-associated kinase and cell cycle-related kinase regulate kinesin-2 motility in Caenorhabditis elegans neuronal cilia. Yi P, Xie C, Ou G. Traffic. 2018 Jul;19(7):522-535. doi: 10.1111/tra.12572. Epub 2018 May 17. PubMed
The cisd gene family regulates physiological germline apoptosis through ced-13 and the canonical cell death pathway in Caenorhabditis elegans. King SD, Gray CF, Song L, Nechushtai R, Gumienny TL, Mittler R, Padilla PA. Cell Death Differ. 2019 Jan;26(1):162-178. doi: 10.1038/s41418-018-0108-5. Epub 2018 Apr 17. PubMed
Streptothricin acetyl transferase 2 (Sat2): A dominant selection marker for Caenorhabditis elegans genome editing. Obinata H, Sugimoto A, Niwa S. PLoS One. 2018 May 9;13(5):e0197128. doi: 10.1371/journal.pone.0197128. eCollection 2018. PubMed

Associated Plasmids

The zinc transporter ZIPT-7.1 regulates sperm activation in nematodes. Zhao Y, Tan CH, Krauchunas A, Scharf A, Dietrich N, Warnhoff K, Yuan Z, Druzhinina M, Gu SG, Miao L, Singson A, Ellis RE, Kornfeld K. PLoS Biol. 2018 Jun 7;16(6):e2005069. doi: 10.1371/journal.pbio.2005069. eCollection 2018 Jun. PubMed
Highly efficient RNAi and Cas9-based auto-cloning systems for C. elegans research. Sturm A, Saskoi E, Tibor K, Weinhardt N, Vellai T. Nucleic Acids Res. 2018 Jun 19. pii: 5040046. doi: 10.1093/nar/gky516. PubMed

Associated Plasmids

OFF-responses of interneurons optimize avoidance behaviors depending on stimulus strength via electrical synapses. Hori S, Oda S, Suehiro Y, Iino Y, Mitani S. PLoS Genet. 2018 Jun 25;14(6):e1007477. doi: 10.1371/journal.pgen.1007477. eCollection 2018 Jun. PubMed

Associated Plasmids

Conserved SUN-KASH Interfaces Mediate LINC Complex-Dependent Nuclear Movement and Positioning. Cain NE, Jahed Z, Schoenhofen A, Valdez VA, Elkin B, Hao H, Harris NJ, Herrera LA, Woolums BM, Mofrad MRK, Luxton GWG, Starr DA. Curr Biol. 2018 Oct 8;28(19):3086-3097.e4. doi: 10.1016/j.cub.2018.08.001. Epub 2018 Sep 20. PubMed
CRELD1 is an evolutionarily-conserved maturational enhancer of ionotropic acetylcholine receptors. D'Alessandro M, Richard M, Stigloher C, Gache V, Boulin T, Richmond JE, Bessereau JL. Elife. 2018 Nov 7;7. pii: 39649. doi: 10.7554/eLife.39649. PubMed
Syncytial germline architecture is actively maintained by contraction of an internal actomyosin corset. Priti A, Ong HT, Toyama Y, Padmanabhan A, Dasgupta S, Krajnc M, Zaidel-Bar R. Nat Commun. 2018 Nov 8;9(1):4694. doi: 10.1038/s41467-018-07149-2. PubMed
Regulation of germ cell development by ARI1 family ubiquitin ligases in C. elegans. Poush JA, Blouin NA, Di Bona KR, Lazetic V, Fay DS. Sci Rep. 2018 Dec 10;8(1):17737. doi: 10.1038/s41598-018-35691-y. PubMed
Adhesive L1CAM-Robo Signaling Aligns Growth Cone F-Actin Dynamics to Promote Axon-Dendrite Fasciculation in C. elegans. Chen CH, Hsu HW, Chang YH, Pan CL. Dev Cell. 2019 Jan 28;48(2):215-228.e5. doi: 10.1016/j.devcel.2018.10.028. Epub 2018 Dec 13. PubMed
Systems Properties and Spatiotemporal Regulation of Cell Position Variability during Embryogenesis. Li X, Zhao Z, Xu W, Fan R, Xiao L, Ma X, Du Z. Cell Rep. 2019 Jan 8;26(2):313-321.e7. doi: 10.1016/j.celrep.2018.12.052. PubMed
SUMO peptidase ULP-4 regulates mitochondrial UPR-mediated innate immunity and lifespan extension. Gao K, Li Y, Hu S, Liu Y. Elife. 2019 Jan 15;8. pii: 41792. doi: 10.7554/eLife.41792. PubMed
Construction of a germline-specific RNAi tool in C. elegans. Zou L, Wu D, Zang X, Wang Z, Wu Z, Chen D. Sci Rep. 2019 Feb 20;9(1):2354. doi: 10.1038/s41598-019-38950-8. PubMed
Ectopic Germ Cells Can Induce Niche-like Enwrapment by Neighboring Body Wall Muscle. Gordon KL, Payne SG, Linden-High LM, Pani AM, Goldstein B, Hubbard EJA, Sherwood DR. Curr Biol. 2019 Mar 4;29(5):823-833.e5. doi: 10.1016/j.cub.2019.01.056. Epub 2019 Feb 21. PubMed
A microRNA switch controls dietary restriction-induced longevity through Wnt signaling. Xu Y, He Z, Song M, Zhou Y, Shen Y. EMBO Rep. 2019 May;20(5). pii: embr.201846888. doi: 10.15252/embr.201846888. Epub 2019 Mar 14. PubMed
NATF (Native And Tissue-Specific Fluorescence): A Strategy for Bright, Tissue-Specific GFP Labeling of Native Proteins in Caenorhabditis elegans. He S, Cuentas-Condori A, Miller DM. Genetics. 2019 Apr 5. pii: genetics.119.302063. doi: 10.1534/genetics.119.302063. PubMed

Associated Plasmids

A Model of Hereditary Sensory and Autonomic Neuropathy Type 1 Reveals a Role of Glycosphingolipids in Neuronal Polarity. Cui M, Ying R, Jiang X, Li G, Zhang X, Zheng J, Tam KY, Liang B, Shi A, Gobel V, Zhang H. J Neurosci. 2019 Jul 17;39(29):5816-5834. doi: 10.1523/JNEUROSCI.2541-18.2019. Epub 2019 May 28. PubMed
Spectrin-based membrane skeleton supports ciliogenesis. Jia R, Li D, Li M, Chai Y, Liu Y, Xie Z, Shao W, Xie C, Li L, Huang X, Chen L, Li W, Ou G. PLoS Biol. 2019 Jul 12;17(7):e3000369. doi: 10.1371/journal.pbio.3000369. eCollection 2019 Jul. PubMed
DAF-16/FOXO promotes taste avoidance learning independently of axonal insulin-like signaling. Nagashima T, Iino Y, Tomioka M. PLoS Genet. 2019 Jul 19;15(7):e1008297. doi: 10.1371/journal.pgen.1008297. eCollection 2019 Jul. PubMed
Translational Regulation of Non-autonomous Mitochondrial Stress Response Promotes Longevity. Lan J, Rollins JA, Zang X, Wu D, Zou L, Wang Z, Ye C, Wu Z, Kapahi P, Rogers AN, Chen D. Cell Rep. 2019 Jul 23;28(4):1050-1062.e6. doi: 10.1016/j.celrep.2019.06.078. PubMed
Disease-associated mutations hyperactivate KIF1A motility and anterograde axonal transport of synaptic vesicle precursors. Chiba K, Takahashi H, Chen M, Obinata H, Arai S, Hashimoto K, Oda T, McKenney RJ, Niwa S. Proc Natl Acad Sci U S A. 2019 Aug 27. pii: 1905690116. doi: 10.1073/pnas.1905690116. PubMed

Associated Plasmids

Gene silencing by double-stranded RNA from C. elegans neurons reveals functional mosaicism of RNA interference. Ravikumar S, Devanapally S, Jose AM. Nucleic Acids Res. 2019 Nov 4;47(19):10059-10071. doi: 10.1093/nar/gkz748. PubMed
A secreted microRNA disrupts autophagy in distinct tissues of Caenorhabditis elegans upon ageing. Zhou Y, Wang X, Song M, He Z, Cui G, Peng G, Dieterich C, Antebi A, Jing N, Shen Y. Nat Commun. 2019 Oct 23;10(1):4827. doi: 10.1038/s41467-019-12821-2. PubMed
henn-1/HEN1 Promotes Germline Immortality in Caenorhabditis elegans. Svendsen JM, Reed KJ, Vijayasarathy T, Montgomery BE, Tucci RM, Brown KC, Marks TN, Nguyen DAH, Phillips CM, Montgomery TA. Cell Rep. 2019 Dec 3;29(10):3187-3199.e4. doi: 10.1016/j.celrep.2019.10.114. PubMed
Epidermal Growth Factor Signaling Promotes Sleep through a Combined Series and Parallel Neural Circuit. Konietzka J, Fritz M, Spiri S, McWhirter R, Leha A, Palumbos S, Costa WS, Oranth A, Gottschalk A, Miller DM 3rd, Hajnal A, Bringmann H. Curr Biol. 2020 Jan 6;30(1):1-16.e13. doi: 10.1016/j.cub.2019.10.048. Epub 2019 Dec 12. PubMed
Epidermal control of axonal attachment via beta-spectrin and the GTPase-activating protein TBC-10 prevents axonal degeneration. Coakley S, Ritchie FK, Galbraith KM, Hilliard MA. Nat Commun. 2020 Jan 9;11(1):133. doi: 10.1038/s41467-019-13795-x. PubMed
Distinct roles of two eIF4E isoforms in the germline of Caenorhabditis elegans. Huggins HP, Subash JS, Stoffel H, Henderson MA, Hoffman JL, Buckner DS, Sengupta MS, Boag PR, Lee MH, Keiper BD. J Cell Sci. 2020 Mar 30;133(6). pii: jcs.237990. doi: 10.1242/jcs.237990. PubMed
Translesion synthesis polymerases are dispensable for C. elegans reproduction but suppress genome scarring by polymerase theta-mediated end joining. van Bostelen I, van Schendel R, Romeijn R, Tijsterman M. PLoS Genet. 2020 Apr 24;16(4):e1008759. doi: 10.1371/journal.pgen.1008759. eCollection 2020 Apr. PubMed
Optimal sidestepping of intraflagellar transport kinesins regulates structure and function of sensory cilia. Xie C, Li L, Li M, Shao W, Zuo Q, Huang X, Chen R, Li W, Brunnbauer M, Okten Z, Chen L, Ou G. EMBO J. 2020 Jun 17;39(12):e103955. doi: 10.15252/embj.2019103955. Epub 2020 Apr 27. PubMed
Modified auxin improves the auxin-inducible degradation (AID) system for laid C. elegans embryos. Negishi T, Asakawa M, Kanemaki M, Sawa H. MicroPubl Biol. 2019 Dec 10;2019. doi: 10.17912/micropub.biology.000190. PubMed
New allele of C. elegans gene pign-1, named as xyz11. Narimatsu T, Ihara S. MicroPubl Biol. 2019 Jan 18;2019. doi: 10.17912/micropub.biology.000088. PubMed
The spectrin-based membrane skeleton is asymmetric and remodels during neural development in C. elegans. Jia R, Chai Y, Xie C, Liu G, Zhu Z, Huang K, Li W, Ou G. J Cell Sci. 2020 Aug 5;133(15). pii: jcs.248583. doi: 10.1242/jcs.248583. PubMed
Caenorhabditis elegans Flamingo FMI-1 controls dendrite self-avoidance through F-actin assembly. Hsu HW, Liao CP, Chiang YC, Syu RT, Pan CL. Development. 2020 Jul 31;147(14). pii: dev.179168. doi: 10.1242/dev.179168. PubMed

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