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pGEX-4T-1 3xHA-MEK1-DD
(Plasmid #47576)

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Full plasmid sequence is not available for this item.

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Item Catalog # Description Quantity Price (USD)
Plasmid 47576 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGEX-4T-1
  • Backbone manufacturer
    Amersham
  • Backbone size w/o insert (bp) 5062
  • Total vector size (bp) 6244
  • Modifications to backbone
    3xHA tag inserted at BamHI site of pGEX-4T-1, regenerating the BamHI site downstream of 3xFlag, with a unique HindIII site added between the BamHI and EcoRI sites of pGEX-4T-1.
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    BL21
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    MEK1-DD
  • Alt name
    MAP2K1
  • Alt name
    MEK1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1182
  • Mutation
    Ser218 to Asp, Ser221 to Asp
  • GenBank ID
    NM_008927
  • Entrez Gene
    Map2k1 (a.k.a. MAPKK1, MEKK1, Mek1, Prkmk1)
  • Tags / Fusion Proteins
    • GST (N terminal on backbone)
    • 3xHA (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer pGEX 5'
  • 3′ sequencing primer pGEX 3'
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Addgene plasmid #15268.
  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX-4T-1 3xHA-MEK1-DD was a gift from Kevin Janes (Addgene plasmid # 47576 ; http://n2t.net/addgene:47576 ; RRID:Addgene_47576)
  • For your References section:

    A high-throughput assay for phosphoprotein-specific phosphatase activity in cellular extracts. Bose AK, Janes KA. Mol Cell Proteomics. 2013 Mar;12(3):797-806. doi: 10.1074/mcp.O112.024059. Epub 2012 Dec 11. 10.1074/mcp.O112.024059 PubMed 23233447