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pCS-PesaRlux
(Plasmid #47640)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 47640 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCS26
  • Backbone manufacturer
    M. Surette (McMaster University)
  • Backbone size w/o insert (bp) 9360
  • Total vector size (bp) 9646
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    PesaR
  • Alt name
    esaR promoter
  • Species
    Pantoea stewartii subsp. stewartii
  • Insert Size (bp)
    286
  • GenBank ID
  • Promoter PesaR

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CCAGCTGGCAATTCCGA
  • 3′ sequencing primer AATCATCACTTTCGGGAA
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    We amplified PesaR from pTDM7 (obtained from S. von Bodman, Minogue et al., 2002, Mol. Microbiol.). We received pCS26 from M. Surette (Beeston et al., 2002, J. Bacteriol.).
  • Terms and Licenses

Depositor Comments

PesaR (274 bp, indicated as lowercase letters in the partial sequence labeled) is located between XhoI and BamHI sites.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCS-PesaRlux was a gift from Cynthia Collins (Addgene plasmid # 47640 ; http://n2t.net/addgene:47640 ; RRID:Addgene_47640)
  • For your References section:

    Directed evolution of the quorum-sensing regulator EsaR for increased signal sensitivity. Shong J, Huang YM, Bystroff C, Collins CH. ACS Chem Biol. 2013 Apr 19;8(4):789-95. doi: 10.1021/cb3006402. Epub 2013 Feb 6. 10.1021/cb3006402 PubMed 23363022