pOTTC352 - pAAV CaMKIIa eNpHR3.0-iRFP
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||47904||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonepAAV-CaMKIIa-eNpHR 3.0-EYFP
Backbone manufacturerKarl Deisseroth
Vector typeMammalian Expression, AAV
Growth in Bacteria
Copy numberHigh Copy
Alt nameengineered halorhodopsin
- Promoter CaMKIIa
/ Fusion Protein
- iRFP (C terminal on insert)
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer CaMKIIa prom F1221 (5'CGTCAGTCAAGCCGGTTCTC)
- 3′ sequencing primer WPRE_R1 (5'ATGAAAGCCATACGGGAAGC) (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made bythe ORF for iRFP was obtained from Addgene (Plasmid 31857). the backbone was obtained from Karl Deisseroth, but is also available from Addgene (Plasmid 26971).
Terms and Licenses
Article Citing this Plasmid
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pOTTC352 - pAAV CaMKIIa eNpHR3.0-iRFP was a gift from Brandon Harvey (Addgene plasmid # 47904)