Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||48250||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
Insert Size (bp)1400
- Promoter CMV
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
pHyper was cloned by replacing EGFP with the Hyper sequence in the pEGFP-C1 vector (Clontech). To make pSypHer, the C199S mutation was made with the QuikChange II site-directed mutagenesis kit using the following oligos:
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:SypHer was a gift from Nicolas Demaurex (Addgene plasmid # 48250 ; http://n2t.net/addgene:48250 ; RRID:Addgene_48250)
For your References section:Dynamic regulation of the mitochondrial proton gradient during cytosolic calcium elevations. Poburko D, Santo-Domingo J, Demaurex N. J Biol Chem. 2011 Apr 1;286(13):11672-84. doi: 10.1074/jbc.M110.159962. Epub 2011 Jan 11. 10.1074/jbc.M110.159962 PubMed 21224385