PurposeBacterial SP crRNA expression: targets SP to protospacer A (TACCATCTCAAGCTTGTTGA), p15A/chloramphenicol
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||48649||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Total vector size (bp) 2755
Growth in Bacteria
Gene/Insert nameBacterial SP crRNA to prototspacer A
Insert Size (bp)56
- Promoter J23100 promoter
- Cloning method Unknown
- 5′ sequencing primer p15A-F
- 3′ sequencing primer CAT-R (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:PM-SP!TA was a gift from George Church (Addgene plasmid # 48649 ; http://n2t.net/addgene:48649 ; RRID:Addgene_48649)
For your References section:Orthogonal Cas9 proteins for RNA-guided gene regulation and editing. Esvelt KM, Mali P, Braff JL, Moosburner M, Yaung SJ, Church GM. Nat Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681. 10.1038/nmeth.2681 PubMed 24076762