|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||48960||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Backbone manufacturerlife technologies
- Backbone size w/o insert (bp) 4959
- Total vector size (bp) 8732
Modifications to backbonea unc-54 3'UTR was cloned to the initial pDEST vector
Growth in Bacteria
- Promoter eef-1A.1 (eft-3)
- Cloning method Gateway Cloning
- 5′ sequencing primer TGCTCActccgtagcagcc
- 3′ sequencing primer aagaaagaagagtgatagagaagaaggg (Common Sequencing Primers)
Please note: eft-3 has officially been changed to eef-1A.1 Please see the eef-1A.1 WormBase entry for details: http://www.wormbase.org/species/c_elegans/gene/WBGene00001168#05-9g-3
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:peft-3::cas-9::tbb-2 3'UTR was a gift from Mario de Bono (Addgene plasmid # 48960 ; http://n2t.net/addgene:48960 ; RRID:Addgene_48960)
For your References section:Efficient genome editing in Caenorhabditis elegans by CRISPR-targeted homologous recombination. Chen C, Fenk LA, de Bono M. Nucleic Acids Res. 2013 Sep 5. 10.1093/nar/gkt805 PubMed 24013562