PurposeExpresses unc-22 sgRNA in C.elegans. Can be used in Co-CRISPR experiments.
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||58202||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonepCR-Blunt II-TOPO
- Backbone size w/o insert (bp) 3519
- Total vector size (bp) 4359
Vector typeWorm Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameunc-22 sgRNA
Alt nameunc-22 guide
SpeciesC. elegans (nematode)
- Promoter U6
- Cloning method TOPO Cloning
- 5′ sequencing primer Sp6
- 3′ sequencing primer T7 (Common Sequencing Primers)
gRNA target sequence GAACCCGTTGCCGAATACAC
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCCM935 was a gift from Craig Mello (Addgene plasmid # 58202 ; http://n2t.net/addgene:58202 ; RRID:Addgene_58202)
For your References section:A Co-CRISPR Strategy for Efficient Genome Editing in Caenorhabditis elegans. Kim H, Ishidate T, Ghanta KS, Seth M, Conte D Jr, Shirayama M, Mello CC. Genetics. 2014 May 30. pii: genetics.114.166389. 10.1534/genetics.114.166389 PubMed 24879462