Purpose(Empty Backbone) FX cloning Pichia pastoris expression vector with AOX1 promoter and N-terminal 10x His tag, GFP and 3C protease cleavage site
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||49021||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size (bp) 5764
Modifications to backbonemodified for compatibility with FX cloning and added tags/fusion proteins
Vector typeYeast Expression
- Promoter AOX1
/ Fusion Proteins
- decaHis (10x His) (N terminal on backbone)
- GFP (N terminal on backbone)
- 3C protease site (PreScission site) (N terminal on backbone)
Growth in Bacteria
Bacterial Resistance(s)Chloramphenicol and Bleocin (Zeocin), 25 & 50 μg/mL
Copy numberLow Copy
- Cloning method Unknown
- 5′ sequencing primer AOX1-fwd (5'-GACTGGTTCCAATTGACAAGC-3')
- 3′ sequencing primer AOX1-rev (5'-GCAAATGGCATTCTGACATCC-3') (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Constructed by Dr. Stephan Schenck, Dutzler lab, Dept of Biochemistry, University of Zurich, Switzerland.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pICXNHG3 was a gift from Raimund Dutzler & Eric Geertsma (Addgene plasmid # 49021 ; http://n2t.net/addgene:49021 ; RRID:Addgene_49021)
For your References section:A versatile and efficient high-throughput cloning tool for structural biology. Geertsma ER, Dutzler R. Biochemistry. 2011 Apr 19;50(15):3272-8. Epub 2011 Mar 25. 10.1021/bi200178z PubMed 21410291