|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||49383||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 7350
- Total vector size (bp) 7368
Modifications to backboneFor cloning oligonucleotides into pmirGLO, bp 7314-7331 were excised (region between PmeI and XbaI).
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameSmad family member 4
SpeciesH. sapiens (human), M. musculus (mouse)
Insert Size (bp)36
Entrez GeneSMAD4 (a.k.a. DPC4, JIP, MADH4, MYHRS)
- Cloning method Restriction Enzyme
- 5′ cloning site PmeI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer GAAGCTGAGTTGGCTGCT
- 3′ sequencing primer CACTGCATTCTAGTTGTGGT (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pmirGLO-Smad4-1719 was a gift from Martine Roussel (Addgene plasmid # 49383 ; http://n2t.net/addgene:49383 ; RRID:Addgene_49383)
For your References section:Silencing of the miR-17~92 cluster family inhibits medulloblastoma progression. Murphy BL, Obad S, Bihannic L, Ayrault O, Zindy F, Kauppinen S, Roussel MF. Cancer Res. 2013 Oct 21. 10.1158/0008-5472.CAN-13-0927 PubMed 24145352