Psicheck2 COLIA1 full length 3'UTR
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||26993||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 6300
Vector typeMammalian Expression, Luciferase
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameCOL1A1 Full length 3' UTR
SpeciesH. sapiens (human)
Insert Size (bp)1400
Entrez GeneCOL1A1 (a.k.a. EDSARTH1, EDSC, OI1, OI2, OI3, OI4)
/ Fusion Protein
- Renilla Luciferase (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer Rluc-F
- 3′ sequencing primer psiCHECK2-R (CGAGGTCCGAAGACTCATTT) (Common Sequencing Primers)
Terms and Licenses
A single base pair mutation is present in the UTR insert
that does not alter the miR-335 complementary motif.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Psicheck2 COLIA1 full length 3'UTR was a gift from Joan Massague (Addgene plasmid # 26993 ; http://n2t.net/addgene:26993 ; RRID:Addgene_26993)
For your References section:Endogenous human microRNAs that suppress breast cancer metastasis. Tavazoie SF, Alarcon C, Oskarsson T, Padua D, Wang Q, Bos PD, Gerald WL, Massague J. Nature. 2008 Jan 10. 451(7175):147-52. 10.1038/nature06487 PubMed 18185580