Holiday Schedule: Addgene will be closed December 22nd & 25th and January 1st. For details, see our holiday shipping schedule. If you have any questions, please contact us at [email protected].
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pBMN HAYFP-LOV24
(Plasmid #49570)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 49570 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBMN
  • Backbone manufacturer
    Garry Nolan
  • Backbone size w/o insert (bp) 6943
  • Total vector size (bp) 8150
  • Vector type
    Retroviral
  • Selectable markers
    HcRed fluorescence behind IRES

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    30°C
  • Growth Strain(s)
    Top10
  • Growth instructions
    TOP10 cells. Culture at 30C to prevent recombination of LTR and lost insert. Takes >20 hours to get good culture at 30C
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    YFP-LOV24
  • Alt name
    YFP fused to B-LID
  • Species
    Avena sativaied from LOV2 domain
  • Insert Size (bp)
    1203
  • Mutation
    delete the last 3 aa from AsLOV2, with I532A mutation and RRRG degron
  • GenBank ID
    AF033096.1
  • Promoter LTR
  • Tags / Fusion Proteins
    • YFP (N terminal on insert)
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site NsiI (not destroyed)
  • 5′ sequencing primer Lab 32
  • 3′ sequencing primer LC47
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBMN HAYFP-LOV24 was a gift from Thomas Wandless (Addgene plasmid # 49570)
  • For your References section:

    A General Method for Regulating Protein Stability with Light. Bonger KM, Rakhit R, Payumo AY, Chen JK, Wandless TJ. ACS Chem Biol. 2013 Nov 1. 10.1021/cb400755b PubMed 24180414