|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||49570||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
Backbone manufacturerGarry Nolan
- Backbone size w/o insert (bp) 6943
- Total vector size (bp) 8150
Selectable markersHcRed fluorescence behind IRES
Growth in Bacteria
Growth instructionsTOP10 cells. Culture at 30C to prevent recombination of LTR and lost insert. Takes >20 hours to get good culture at 30C
Copy numberLow Copy
Alt nameYFP fused to B-LID
SpeciesAvena sativaied from LOV2 domain
Insert Size (bp)1203
Mutationdelete the last 3 aa from AsLOV2, with I532A mutation and RRRG degron
- Promoter LTR
/ Fusion Proteins
- YFP (N terminal on insert)
- HA (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site NsiI (not destroyed)
- 5′ sequencing primer Lab 32
- 3′ sequencing primer LC47 (Common Sequencing Primers)
Terms and Licenses
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBMN HAYFP-LOV24 was a gift from Thomas Wandless (Addgene plasmid # 49570)
For your References section:A General Method for Regulating Protein Stability with Light. Bonger KM, Rakhit R, Payumo AY, Chen JK, Wandless TJ. ACS Chem Biol. 2013 Nov 1. 10.1021/cb400755b PubMed 24180414
Generated by Addgene from full sequence supplied by depositor.
Map uploaded by the depositor.