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pGP-CMV-GCaMP6s-CAAX
(Plasmid #52228)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 52228 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEGFP-N1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4005
  • Total vector size (bp) 5436
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GCaMP6s-CAAX
  • Species
    Synthetic
  • Insert Size (bp)
    1431
  • Promoter CMV
  • Tags / Fusion Proteins
    • 6xHis (N terminal on insert)
    • CAAX (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer CMV Forward
  • 3′ sequencing primer SV40pA-R
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid

Depositor Comments

GCaMP6s (Addgene plasmid 40753) tagged with CAAX motif (amino acids KEKMSKDGKKKKKKSKTKCVIM)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGP-CMV-GCaMP6s-CAAX was a gift from Tobias Meyer (Addgene plasmid # 52228 ; http://n2t.net/addgene:52228 ; RRID:Addgene_52228)
  • For your References section:

    A polarized Ca(2+), diacylglycerol and STIM1 signalling system regulates directed cell migration. Tsai FC, Seki A, Yang HW, Hayer A, Carrasco S, Malmersjo S, Meyer T. Nat Cell Biol. 2014 Feb;16(2):133-44. doi: 10.1038/ncb2906. Epub 2014 Jan 26. 10.1038/ncb2906 PubMed 24463606