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pGL3E-hGPR56 e1m promoter perisylvian polymicrogyria
(Plasmid #52299)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 52299 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGL3E
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 5064
  • Total vector size (bp) 5370
  • Vector type
    Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ADGRG1 adhesion G protein-coupled receptor G1
  • Species
    H. sapiens (human)
  • Mutation
    15-bp deletion in the promoter
  • Entrez Gene
    ADGRG1 (a.k.a. BFPP, BPPR, GPR56, TM7LN4, TM7XN1)
  • Promoter Human GPR56 e1m promoter

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer RV3
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGL3E-hGPR56 e1m promoter perisylvian polymicrogyria was a gift from Christopher A Walsh (Addgene plasmid # 52299 ; http://n2t.net/addgene:52299 ; RRID:Addgene_52299)
  • For your References section:

    Evolutionarily dynamic alternative splicing of GPR56 regulates regional cerebral cortical patterning. Bae BI, Tietjen I, Atabay KD, Evrony GD, Johnson MB, Asare E, Wang PP, Murayama AY, Im K, Lisgo SN, Overman L, Sestan N, Chang BS, Barkovich AJ, Grant PE, Topcu M, Politsky J, Okano H, Piao X, Walsh CA. Science. 2014 Feb 14;343(6172):764-8. doi: 10.1126/science.1244392. 10.1126/science.1244392 PubMed 24531968