pGL3E-hGPR56 e1m promoter normal
PurposeReporter for normal human GPR56 exon 1m promoter activity. Human GPR56 e1m promoter (chr16:56,230,630-56,230,943 (hg18)) was inserted into pGL3E.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||52298||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5064
- Total vector size (bp) 5385
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameADGRG1 adhesion G protein-coupled receptor G1
SpeciesH. sapiens (human)
Entrez GeneADGRG1 (a.k.a. BFPP, BPPR, GPR56, TM7LN4, TM7XN1)
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer RV3 (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pGL3E-hGPR56 e1m promoter normal was a gift from Christopher A Walsh (Addgene plasmid # 52298 ; http://n2t.net/addgene:52298 ; RRID:Addgene_52298)
For your References section:Evolutionarily dynamic alternative splicing of GPR56 regulates regional cerebral cortical patterning. Bae BI, Tietjen I, Atabay KD, Evrony GD, Johnson MB, Asare E, Wang PP, Murayama AY, Im K, Lisgo SN, Overman L, Sestan N, Chang BS, Barkovich AJ, Grant PE, Topcu M, Politsky J, Okano H, Piao X, Walsh CA. Science. 2014 Feb 14;343(6172):764-8. doi: 10.1126/science.1244392. 10.1126/science.1244392 PubMed 24531968