Purposehuman ErbB-3 promoter reporter construct
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||60899||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4818
- Total vector size (bp) 5936
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameErbB-3 promoter
SpeciesH. sapiens (human)
Insert Size (bp)1118
Entrez GeneERBB3 (a.k.a. ErbB-3, FERLK, HER3, LCCS2, MDA-BF-1, c-erbB-3, c-erbB3, erbB3-S, p180-ErbB3, p45-sErbB3, p85-sErbB3)
/ Fusion Protein
- luciferase (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer RVprimer3 (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:ErbB-3-pGL3 was a gift from Frederick Domann (Addgene plasmid # 60899 ; http://n2t.net/addgene:60899 ; RRID:Addgene_60899)
For your References section:Dominant negative interference of transcription factor AP-2 causes inhibition of ErbB-3 expression and suppresses malignant cell growth. Zhu CH, Domann FE. Breast Cancer Res Treat. 2002 Jan;71(1):47-57. PubMed 11859873