This website uses cookies to ensure you get the best experience. By continuing the use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pLVE-hTGFB3-IRES-Red
(Plasmid #52580)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 52580 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pLV-tTRKRAB-red
  • Backbone size w/o insert (bp) 10527
  • Total vector size (bp) 12293
  • Modifications to backbone
    Added MCS downstream of EF-1alpha promoter in backbone prior to cloning.
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    human transforming growth factor-beta3
  • Alt name
    TGF-beta3
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1239
  • GenBank ID
    NM_003239.2
  • Entrez Gene
    TGFB3 (a.k.a. ARVD, ARVD1, LDS5, RNHF, TGF-beta3)
  • Promoter EF-1alpha

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site MluI (not destroyed)
  • 5′ sequencing primer CCA GCT TGG CAC TTG ATG TA
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLVE-hTGFB3-IRES-Red was a gift from Charles Gersbach (Addgene plasmid # 52580)
  • For your References section:

    Scaffold-mediated lentiviral transduction for functional tissue engineering of cartilage. Brunger JM, Huynh NP, Guenther CM, Perez-Pinera P, Moutos FT, Sanchez-Adams J, Gersbach CA, Guilak F. Proc Natl Acad Sci U S A. 2014 Mar 4;111(9):E798-806. doi: 10.1073/pnas.1321744111. Epub 2014 Feb 18. 10.1073/pnas.1321744111 PubMed 24550481