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pGEX4T2-Ste7-EE
(Plasmid #52681)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 52681 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGEX-4T2
  • Backbone manufacturer
    GE Healthcare Life Sciences
  • Backbone size w/o insert (bp) 4900
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Ste7
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    1545
  • Mutation
    S359E/T363E constitutively active
  • GenBank ID
    851396
  • Entrez Gene
    STE7 (a.k.a. YDL159W)
  • Promoter tac
  • Tags / Fusion Proteins
    • GST (N terminal on backbone)
    • Myc (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer pGEX5'
  • 3′ sequencing primer pGEX3'
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Beverly Errede (please see Maleri et al, Mol Cell Biol. 2004 Oct;24(20):9221-38. PubMed: 15456892 )
  • Terms and Licenses

Depositor Comments

A F2V mutation was discovered, it should not effect the function of the plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX4T2-Ste7-EE was a gift from Benjamin Turk (Addgene plasmid # 52681 ; http://n2t.net/addgene:52681 ; RRID:Addgene_52681)
  • For your References section:

    Deciphering protein kinase specificity through large-scale analysis of yeast phosphorylation site motifs. Mok J, Kim PM, Lam HY, Piccirillo S, Zhou X, Jeschke GR, Sheridan DL, Parker SA, Desai V, Jwa M, Cameroni E, Niu H, Good M, Remenyi A, Ma JL, Sheu YJ, Sassi HE, Sopko R, Chan CS, De Virgilio C, Hollingsworth NM, Lim WA, Stern DF, Stillman B, Andrews BJ, Gerstein MB, Snyder M, Turk BE. Sci Signal. 2010 Feb 16;3(109):ra12. doi: 10.1126/scisignal.2000482. 10.1126/scisignal.2000482 PubMed 20159853