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AaHsp70Bb-1696-FFluc
(Plasmid #52912)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 52912 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGL3
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 4773
  • Total vector size (bp) 6650
  • Vector type
    Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Aa Hsp70B-1696
  • Species
    Aedes aegypti
  • Insert Size (bp)
    1872
  • Promoter Aa Hsp70B-1696

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site MluI (destroyed during cloning)
  • 3′ cloning site NcoI (not destroyed)
  • 5′ sequencing primer ataggctgtccccagtgcaagt
  • 3′ sequencing primer tttcatagcttctgccaaccgaacgg
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AaHsp70Bb-1696-FFluc was a gift from Zach Adelman (Addgene plasmid # 52912 ; http://n2t.net/addgene:52912 ; RRID:Addgene_52912)
  • For your References section:

    Identification and characterization of heat shock 70 genes in Aedes aegypti (Diptera: Culicidae). Gross TL, Myles KM, Adelman ZN. J Med Entomol. 2009 May;46(3):496-504. PubMed 19496419