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PurposeRecombinational donor/master vector
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 53446 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * | |
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Backbone
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Vector backboneGateway pDONR 221
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Backbone manufacturerLife Technologies
- Backbone size w/o insert (bp) 4761
- Total vector size (bp) 10677
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Vector typeGateway
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature30°C
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Growth Strain(s)Stbl2
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Copy numberUnknown
Gene/Insert
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Gene/Insert name53BP1
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SpeciesH. sapiens (human)
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Insert Size (bp)5916
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MutationsiRNA resistant and mutations A128V, T720A, A1347V, N1632S. Please see depositor's comments below.
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Entrez GeneTP53BP1 (a.k.a. 53BP1, TDRD30, p202, p53BP1)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note that mutations A128V, T720A, A1347V, N1632S in 53BP1 were found during Addgene's quality control. The depositor noted that these mutations do NOT affect plasmid function and 53BP1 functions as wild type.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDONR-53BP1 1-1972 WT was a gift from Daniel Durocher (Addgene plasmid # 53446 ; http://n2t.net/addgene:53446 ; RRID:Addgene_53446) -
For your References section:
53BP1 is a reader of the DNA-damage-induced H2A Lys 15 ubiquitin mark. Fradet-Turcotte A, Canny MD, Escribano-Diaz C, Orthwein A, Leung CC, Huang H, Landry MC, Kitevski-LeBlanc J, Noordermeer SM, Sicheri F, Durocher D. Nature. 2013 Jul 4;499(7456):50-4. doi: 10.1038/nature12318. Epub 2013 Jun 12. 10.1038/nature12318 PubMed 23760478
