PurposeLocalization: Focal Adhesions, Excitation: 490 / 553, Emission: 507 / 573
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||57749||Standard format: Plasmid sent in bacteria as agar stab||1||$75 *|
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- Backbone size w/o insert (bp) 4750
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Entrez GeneVCL (a.k.a. CMD1W, CMH15, HEL114, MV, MVCL)
- Promoter CMV
/ Fusion Protein
- Dendra2 (C terminal on backbone)
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
. Excitation = 490 / 553; Emission = 507 / 573
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Dendra2-Vinculin-N-21 was a gift from Michael Davidson (Addgene plasmid # 57749 ; http://n2t.net/addgene:57749 ; RRID:Addgene_57749)
For your References section:Interferometric fluorescent super-resolution microscopy resolves 3D cellular ultrastructure. Shtengel G, Galbraith JA, Galbraith CG, Lippincott-Schwartz J, Gillette JM, Manley S, Sougrat R, Waterman CM, Kanchanawong P, Davidson MW, Fetter RD, Hess HF. Proc Natl Acad Sci U S A. 2009 Mar 3;106(9):3125-30. doi: 10.1073/pnas.0813131106. Epub 2009 Feb 6. 10.1073/pnas.0813131106 PubMed 19202073