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VV020: WT Arch-3 - eGFP in pET28b
(Plasmid #58488)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 58488 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET28b
  • Backbone size w/o insert (bp) 5269
  • Total vector size (bp) 6772
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Growth instructions
    Express in BL21(DE3); See attached document or Maclaurin et al 2013 [PMID 23530193] for expression instructions.
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Archaerhodopsin-3
  • Alt name
    Arch
  • Species
    Halorubrum sodomense
  • Insert Size (bp)
    1503
  • GenBank ID
    BAA09452.1
  • Promoter T7
  • Tag / Fusion Protein
    • eGFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer TAA TAC GAC TCA CTA TAG GG
  • 3′ sequencing primer GCT AGT TAT TGC TCA GCG G
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    VV020: WT Arch-3 - eGFP in pET28b was a gift from Adam Cohen (Addgene plasmid # 58488)
  • For your References section:

    Mechanism of voltage-sensitive fluorescence in a microbial rhodopsin. Maclaurin D, Venkatachalam V, Lee H, Cohen AE. Proc Natl Acad Sci U S A. 2013 Apr 9;110(15):5939-44. doi: 10.1073/pnas.1215595110. Epub 2013 Mar 25. 10.1073/pnas.1215595110 PubMed 23530193