PurposeFluorescent reporter for membrane voltage with improved brightness
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||64135||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3974
- Total vector size (bp) 4754
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
Insert Size (bp)780
- Cloning method Restriction Enzyme
- 5′ cloning site XBAI (not destroyed)
- 3′ cloning site HINDIII (not destroyed)
- 5′ sequencing primer TGGGCTAACAGGAGGAATTAAC
- 3′ sequencing primer TGGTGAGCAAGGGCTAGAAG (Common Sequencing Primers)
The N and C termini differ slightly from the mammalian versions of QuasAr1 (Addgene 51629) and QuasAr2 (Addgene 51692), but these differences are not believed to be functionally significant.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBAD_His B_QuasAr1 was a gift from Adam Cohen (Addgene plasmid # 64135 ; http://n2t.net/addgene:64135 ; RRID:Addgene_64135)
For your References section:All-optical electrophysiology in mammalian neurons using engineered microbial rhodopsins. Hochbaum DR, Zhao Y, Farhi SL, Klapoetke N, Werley CA, Kapoor V, Zou P, Kralj JM, Maclaurin D, Smedemark-Margulies N, Saulnier JL, Boulting GL, Straub C, Cho YK, Melkonian M, Wong GK, Harrison DJ, Murthy VN, Sabatini BL, Boyden ES, Campbell RE, Cohen AE. Nat Methods. 2014 Aug;11(8):825-33. doi: 10.1038/nmeth.3000. Epub 2014 Jun 22. 10.1038/nmeth.3000 PubMed 24952910