All-optical electrophysiology in mammalian neurons using engineered microbial rhodopsins.
Hochbaum DR, Zhao Y, Farhi SL, Klapoetke N, Werley CA, Kapoor V, Zou P, Kralj JM, Maclaurin D, Smedemark-Margulies N, Saulnier JL, Boulting GL, Straub C, Cho YK, Melkonian M, Wong GK, Harrison DJ, Murthy VN, Sabatini BL, Boyden ES, Campbell RE, Cohen AE
Nat Methods. 2014 Aug;11(8):825-33. doi: 10.1038/nmeth.3000. Epub 2014 Jun 22. PubMed Article
Hochbaum DR, Zhao Y, Farhi SL, Klapoetke N, Werley CA, Kapoor V, Zou P, Kralj JM, Maclaurin D, Smedemark-Margulies N, Saulnier JL, Boulting GL, Straub C, Cho YK, Melkonian M, Wong GK, Harrison DJ, Murthy VN, Sabatini BL, Boyden ES, Campbell RE, Cohen AE
Nat Methods. 2014 Aug;11(8):825-33. doi: 10.1038/nmeth.3000. Epub 2014 Jun 22. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 51629 | DRH229: FCK-QuasAr1-mO2 | Fluorescent reporter for membrane voltage with improved brightness |
| 51692 | DRH334: FCK-QuasAr2-mO2 | Fluorescent reporter of membrane voltage with improved sensitivity |
| 51693 | DRH313: FCK-CheRiff-eGFP | Channelrhodopsin with improved sensitivity and minimal red light photocurrent |
| 51694 | DRH296: FCK-Optopatch2 | Coexpression plasmid of QuasAr2 and CheRiff for all-optical electrophysiology |
| 51695 | DRH335: FCK-Optopatch1 | Coexpression plasmid of QuasAr1 and CheRiff for all-optical electrophysiology |
| 51696 | DRH322: AAV-CaMKIIa-QuasAr2-mO2 | AAV production of QuasAr2 fluorescent reporter |
| 51697 | DRH337: AAV-hsyn-CheRiff-eGFP | AAV production of CheRiff channelrhodopsin actuator |
| 62984 | pMOS001: CMV-Optopatch2_FCK | Coexpression plasmid of QuasAr2 and CheRiff for all-optical electrophysiology under CMV promoter |
| 62985 | pMOS009: pLX304-QuasAr2 | Lentivirus production of QuasAr2 fluorescent reporter |
| 62986 | pMOS010: pLX304-CheRiff-GFP | Lentivirus production of CheRiff channelrhodopsin actuator |
| 64134 | pBAD_His B_QuasAr2 | Fluorescent reporter of membrane voltage with improved sensitivity |
| 64135 | pBAD_His B_QuasAr1 | Fluorescent reporter for membrane voltage with improved brightness |