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DRH337: AAV-hsyn-CheRiff-eGFP
(Plasmid #51697)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 51697 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pAAV
  • Backbone size w/o insert (bp) 4281
  • Total vector size (bp) 6015
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Growth instructions
    Use recombinase-free E. coli (e.g., Invitrogen's Stbl3)
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CheRiff-eGFP
  • Alt name
    ChR64
  • Species
    Synthetic
  • Insert Size (bp)
    1734
  • Promoter hSynapsin
  • Tag / Fusion Protein
    • eGFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer TGAGAGCGCAGTCGAGAAG
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    DRH337: AAV-hsyn-CheRiff-eGFP was a gift from Adam Cohen (Addgene plasmid # 51697)
  • For your References section:

    All-optical electrophysiology in mammalian neurons using engineered microbial rhodopsins. Hochbaum DR, Zhao Y, Farhi SL, Klapoetke N, Werley CA, Kapoor V, Zou P, Kralj JM, Maclaurin D, Smedemark-Margulies N, Saulnier JL, Boulting GL, Straub C, Cho YK, Melkonian M, Wong GKS, Harrison DJ, Murthy VN, Sabatini BL, Boyden ES, Campbell RE & Cohen AE. Nature Methods (2014) doi:10.1038/nmeth.3000 10.1038/nmeth.3000