pOTTC588 - pAAV c-fos Nuc-iRFP
PurposeAn AAV vector that expresses nuclear localized iRFP under control of the c-fos promoter.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||59132||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression, AAV
Growth in Bacteria
Growth Strain(s)NEB Stable
Copy numberLow Copy
Gene/Insert nameNuclear-localized Infrared Fluorescent Protein
- Promoter mFos
/ Fusion Protein
- 3xNLS (C terminal on insert)
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer mFos prom F1098 AGGACTTGTCTGAGCGCGTG
- 3′ sequencing primer WPRE R1 (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pOTTC588 - pAAV c-fos Nuc-iRFP was a gift from Brandon Harvey (Addgene plasmid # 59132 ; http://n2t.net/addgene:59132 ; RRID:Addgene_59132)
For your References section:Near-infrared fluorescent protein iRFP713 as a reporter protein for optogenetic vectors, a transgenic Cre-reporter rat, and other neuronal studies. Richie CT, Whitaker LR, Whitaker KW, Necarsulmer J, Baldwin HA, Zhang Y, Fortuno L, Hinkle JJ, Koivula P, Henderson MJ, Sun W, Wang K, Smith JC, Pickel J, Ji N, Hope BT, Harvey BK. J Neurosci Methods. 2017 Jun 1;284:1-14. doi: 10.1016/j.jneumeth.2017.03.020. Epub 2017 Apr 2. 10.1016/j.jneumeth.2017.03.020 PubMed 28380331