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pBlu/gRNA
(Plasmid #59188)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 59188 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBluescript KS(+)
  • Backbone size w/o insert (bp) 2958
  • Total vector size (bp) 3515
  • Modifications to backbone
    Added a gRNA cassette with an Arabidopsis U6 promoter and terminator
  • Vector type
    Plant Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Guide RNA Cassette
  • Alt name
    gRNA
  • Species
    A. thaliana (mustard weed)
  • Insert Size (bp)
    551
  • Promoter U6 (Arabidopis)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T3
  • 3′ sequencing primer T5
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBlu/gRNA was a gift from Robert Stupar (Addgene plasmid # 59188 ; http://n2t.net/addgene:59188 ; RRID:Addgene_59188)
  • For your References section:

    CRISPR/Cas mutagenesis of soybean and Medicago truncatula using a new web-tool and a modified Cas9 enzyme. Michno JM, Wang X, Liu J, Curtin SJ, Kono TJ, Stupar RM. GM Crops Food. 2015;6(4):243-52. doi: 10.1080/21645698.2015.1106063. 10.1080/21645698.2015.1106063 PubMed 26479970